Composite

Part:BBa_K539151:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)

promoter+crtEBI+RNA thermometer(FourU)+crtY+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2017
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1553
    Illegal NgoMIV site found at 1683
    Illegal NgoMIV site found at 4654
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our experiment, the order of intensity of RNA thermometer are 37°C, 42°C, 32°C, 27°C. With the help of RNA thermometer, we can control the color of pigment by different temperatures. When the temperature is lower than 37°C, the pigment is red because the translation of crtEBI. When the temperature is higher than 37°C, the pigment is orange because of the translation of crtY that convert lycopene to orange beta-carotene.

Source

Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100) comes from team Peking 2010. Other parts come from parts-registry.

References